Aloysius Wong

OFFICE: GEH B404
EMAIL: aloysiusw@wku.edu.cn

PREVIOUS EMPLOYMENT:
2016 Postdoctoral Researcher
Institute for Integrative Biology of the Cell (I2BC)
French National Centre for Scientific Research (CNRS) campus, France
2014-2016 Postdoctoral Fellow
King Abdullah University of Science and Technology (KAUST), Saudi Arabia

EDUCATIONAL BACKGROUND:
PhD in Bioscience (2011-2014)
King Abdullah University of Science and Technology (KAUST), Saudi Arabia
MPhil in Biotechnology (2010-2011)
University of Cambridge, UK
BSc (Hons) in Biotechnology (2006-2009)
UCSI University, Malaysia

RESEARCH INTEREST:
Dr. Wong seeks to understand from a molecular perspective, how plants despite being sessile and immobile, can efficiently perceive environmental (biotic and abiotic) signals and mount short- and long-term biological responses that enables them to survive in environments considered hostile to plants. His current research can be divided into three main areas: 1) Sustainable and indoor horticulture, 2) Probiotics and antibiotic resistance and, 3) Plant cell signaling.

RECENT PUBLICATIONS:
Journal Articles
1. Al-Younis I, Wong A, Lemtiri-Chlieh F, Schmӧkel S, Tester M, Gehring C & Donaldson L (2018). The Arabidopsis thaliana K+-uptake permease 5 (AtKUP5) contains a functional cytosolic adenylate cyclase essential for K+ transport. Front Plant Sci (accepted)
2. Marondedze C, Liu X, Huang S, Wong C, Zhou X, Pan X, An H, Xu N, Tian X & Wong A (2018). Towards a tailored indoor horticulture: A functional genomics guided phenotypic approach. Hortic Res 2018(5), 68 doi: 10.1038/s41438-018-0065-7
3. Chatukuta P, Dikobe TB, Kawadza DT, Sehlabane KS, Takundwa MM, Wong A, Gehring C & Ruzvidzo O (2018). An Arabidopsis clathrin assembly with a predicted role in plant defense can function as an adenylate cyclase. Biomolecules 23(2), E15, doi: 10.3390/biom8020015
4. Wong A*, Tian X, Gehring C & Marondedze C (2018). Discovery of novel functional centers with rationally designed amino acid motifs. Comput Struct Biotechnol J 16(2018), 70-76 *First & Co-responding author
5. Xu N, Fu D, Li S, Wang Y & Wong A (2018). GCPred: a web tool for guanylyl cyclase functional centre prediction from amino acid sequence. Bioinformatics 34(12), 2134-2135
6. Ooi A*, Lemtiri-Chlieh F*, Wong A & Gehring C (2017). Abscisic acid regulates the conductance of the guard cell outward rectifying K+-channel. Mol Plant 10(11), 1469-1472
7. Zheng M*, Zhang R*, Tian X, Zhou X, Pan X & Wong A (2017). Assessing the risk of probiotic dietary supplements in the context of antibiotic resistance. Front Microbiol 8, 908 *Co-first author
8. Wheeler J, Wong A, Marondedze C, Arnoud G, Kwezi L, Freihat L, Jignesh V, Misjudeen R, Irving H & Gehring C (2017). The brassinosteroid receptor BRI1 can generate cGMP enabling cGMP-dependent downstream signaling. Plant J 91(4), 590-600
9. Ooi A, Wong A, Esau L, Lemtiri-Chlieh F & Gehring C (2016). A guide to transient expression of membrane proteins in HEK-293 cells for electrophysiological characterization. Front Physiol 7, 300
10. Ooi A*, Wong A*, Ng TK, Marondedze C, Gehring C & Ooi BS (2016). Growth and development of Arabidopsis thaliana under single-wavelength red and blue laser light. Sci Rep 6: 33885 *Joint-first author
11. Al-Younis I, Wong A & Gehring C (2015). The Arabidopsis thaliana K+-uptake permease 7 (AtKUP7) contains a functional cytosolic adenylate cyclase catalytic centre. FEBS Lett 589 (24 Part B), 3848-3852
12. Wong A, Gehring C & Irving HR (2015). Conserved functional motifs and homology modeling to predict hidden moonlighting functional sites. Front Bioeng Biotechnol 82, 1-8
13. Wong A, Ngu DYS, Dan LA, Ooi ASL & Lim RLH (2015). Detection of antibiotic resistance in probiotics of dietary supplements. Nutr J 14, 95
14. Domingos P, Prado, AM, Wong A, Gehring C & Feijo J (2014). Nitric oxide: A multitasking gas in plants. Mol Plant 8(4), 506-520
15. Marondedze C, Wong A, Groen AJ, Serrano N, Jankovic B, Lilley KS, Gehring C & Thomas L (2014). Exploring the Arabidopsis Proteome: Influence of protein solubilization buffers on proteome coverage. Int J Mol Sci 16(1), 857-870
16. Wong A & Gehring C (2013). The Arabidopsis thaliana proteome harbors undiscovered multi-domain molecules with functional guanylyl cyclase catalytic centers. Cell Commun Signal 11(1), 48

CONFERENCE PROCEEDINGS:
1. Xu N, Zhang C, Lim LL & Wong A (2018). Bioinformatic analysis of nucleotide cyclase functional centers and development of ACPred webserver. Proceedings of the 2018 ACM International Conference on Bioinformatics, Computational Biology, and Health Informatics (BCB’18). August 29-September 1, 2018, Washington DC, USA
2. Lu L, Michelena T, Wong A, Zhang C & Meng Y (2018). The inhibition of acetylcholinesterase by a brain-targeting polylysine-apoE peptide: biochemical and structural characterizations. Proceedings of the 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (IEEE EMBC’18). July 17-21, 2018, Honolulu HI, USA

BOOK CHAPTERS:
1. Wong A, Thomas L, Gehring C & Marondedze C (2016). Proteomics of fruit development and ripening. In Sunil Pareek (Ed.), Postharvest Ripening Physiology of Crops: CRC Press/Taylor & Francis, 413-448
2. Marondedze C, Wong A, Thomas L & Gehring C (2016). Non-canonical cyclic mono-nucleotides in plants. In R. Seifert (Ed.), From canonical to non-canonical cyclic nucleotides as second messengers: Pharmacological Implications, Handbook of Experimental Pharmacology: Springer Berlin Heidelberg, Switzerland 1-17 doi: 10.1007/164_2015_35
3. Wong A & Gehring C (2013). Computational identification of candidate nucleotide cyclases in higher plants. In C. Gehring (Ed.), Cyclic Nucleotide Signaling in Plants, Methods Mol Biol 1016, 195-205, Humana Press

PATENT:
Laser-based agriculture system, Ooi BS, Wong A & Ng TK. US 14/868,422; US20160088804 A1, March 31, 2016.

GRANTS:
1. National Natural Science Foundation of China (Grant No. 31850410470)
Category: International (Regional) Cooperation and Exchange Project
Project Title: Towards a highly tailored indoor horticulture: A functional genomics guided phenotypic approach
Duration: 2019-01-01 to 2020-12-31
2. Zhejiang Provincial Natural Science Foundation of China (Grant no. Q19C130001)
Category: Youth Science Fund Project
Project Title: 高级定制的室内园艺:一种功能基因组学引导的表现型途径
Duration: 2019-01-01 to 2021-12-31
3. Student partnering with Faculty (SpF) II & III (Grant No. WKU201617002 & WKU201718009)
Project Title: Antibiotic resistant probiotics in dietary and health supplements
Duration: 2016-01-11 to 2019-01-03

AWARDS:
1. Wenzhou-Kean University Student Research Days Keynote Speaker (04-2018)
2. Young Investigator Award by Young Scientist Network Malaysia & Malaysia Academy of Sciences (06-2015)
3. DOW Chemicals Sustainability Innovation Student Challenge (DOW SISCA) Award Winner (10-2013)
4. KAUST PhD Fellowship and Provost Awards (09-2011)

WEBSITES:
ResearchGate
https://www.researchgate.net/profile/Aloysius_Wong
Google Scholar
https://scholar.google.com/citations?user=w434nIEAAAAJ&hl=en
Linkedin
https://www.linkedin.com/in/aloysius-wong-67484819